SPHERO™ Rainbow Kalibrasyon Boncukları
The Rainbow calibration particles, 8 peaks are designed for routine calibration of flow cytometers. Rainbow calibration particles, 8 peaks are commonly used in many laboratories for Quality Control and long-term performance of the analyser.
Rainbow calibration particles, 8 peaks contain several similar sized particles that are dyed in eight different fluorescent intensities. Each particle contains a mixture of fluorophores that are excited at any wavelength from 365 to 650 nm. The same set of particles enables the calibration of all channels in the flow cytometer. The Rainbow Particles have emission spectra compatible with many common fluorochromes used for immunofluorescent staining.
Rainbow calibration particles, 8 peaks (Rainbow calibration, 8 peaks Cat. No. RCP-30-5A) are used in EuroFlow™ standardization guidelines throughout the study of initial PMT characterization, to set target MFI values and daily performance tracking of the flow cytometers.
- EuroFlow™ reference target values (7th peak) for FACSCanto™ II
Batch EAB01, EAC01, EAD05, EAE01, EAF01, EAG01 (check values)
| Lower MFI (-15%) | Rainbow 7-peak (DiVa) | Upper MFI (+15%) | |
| Pacific Blue™ | 100.452 | 118.178 | 135.905 |
| Pacific Orange | 93.871 | 110.436 | 127.002 |
| FITC | 28.752 | 33.826 | 38.900 |
| PE | 32.381 | 38.095 | 43.809 |
| PerCP | 66.846 | 78.642 | 90.438 |
| PC7 | 8.316 | 9.783 | 11.250 |
| APC | 158.639 | 186.634 | 214.629 |
| APC-H7 | 64.194 | 75.522 | 86.850 |
FACSCanto™ is a trademark of Becton, Dickinson and Company. Pacific Blue™ and Pacific Orange™ are trademarks of Life Technologies Corporation. Cy™ is a trademark of GE Healthcare.
EuroFlow™ is a trademark of European Scientific foundation for Laboratory Hemato Oncology (ESLHO).
EuroFlow™ reference target values (7th peak) for Navios™ or Gallios™
Batch EAB01, EAC01, EAD05, EAE01, EAF01; EAG01 (check values)
| Lower MFI (-15%) | Rainbow 7-peak (Navios) | Upper MFI (+15%) | |
| FL9 – Pacific Blue™ | 379,874 | 446,911 | 513,948 |
| FL10 – OC515 | 278,720 | 327,906 | 377,092 |
| FL1 – FITC | 117,719 | 138,493 | 159,267 |
| FL2 – PE | 101,813 | 119,780 | 137,747 |
| FL4 – PerCPCy™5.5 | 253,075 | 297,735 | 342,395 |
| FL5 – PECy™7 | 22,648 | 26,645 | 30,642 |
| FL6 – APC | 637,655 | 750,182 | 862,709 |
| AFL8 – APC-C750 | 162,326 | 190,972 | 219,618 |
Navios™ and Gallios™ are trademarks of Beckman Coulter Inc. Pacific Blue™ is a trademark of Life Technologies. Cy™ is a trademark of GE Healthcare.
EuroFlow™ is a trademark of European Scientific foundation for Laboratory Hemato Oncology (ESLHO).
