SPHERO™ Rainbow Kalibrasyon Boncukları

The Rainbow calibration particles, 8 peaks are designed for routine calibration of flow cytometers. Rainbow calibration particles, 8 peaks are commonly used in many laboratories for Quality Control and long-term performance of the analyser.

Rainbow calibration particles, 8 peaks contain several similar sized particles that are dyed in eight different fluorescent intensities.  Each particle contains a mixture of fluorophores that are excited at any wavelength from 365 to 650 nm. The same set of particles enables the calibration of all channels in the flow cytometer. The Rainbow Particles have emission spectra compatible with many common fluorochromes used for immunofluorescent staining.

Rainbow calibration particles, 8 peaks (Rainbow calibration, 8 peaks Cat. No. RCP-30-5A) are used in EuroFlow™ standardization guidelines throughout the study of initial PMT characterization, to set target MFI values and daily performance tracking of the flow cytometers.

  • EuroFlow™ reference target values (7th peak) for FACSCanto™ II

Batch EAB01, EAC01, EAD05, EAE01, EAF01, EAG01 (check values)

Lower MFI (-15%) Rainbow 7-peak (DiVa) Upper MFI (+15%)
Pacific Blue™ 100.452 118.178 135.905
Pacific Orange 93.871 110.436 127.002
FITC 28.752 33.826 38.900
PE 32.381 38.095 43.809
PerCP 66.846 78.642 90.438
PC7 8.316 9.783 11.250
APC 158.639 186.634 214.629
APC-H7 64.194 75.522 86.850

FACSCanto™ is a trademark of Becton, Dickinson and Company. Pacific Blue™ and Pacific Orange™ are trademarks of Life Technologies Corporation. Cy™ is a trademark of GE Healthcare.
EuroFlow™ is a trademark of European Scientific foundation for Laboratory Hemato Oncology (ESLHO).

EuroFlow™ reference target values (7th peak) for Navios™ or Gallios™ 

Batch EAB01, EAC01, EAD05, EAE01, EAF01; EAG01 (check values)

Lower MFI (-15%) Rainbow 7-peak (Navios) Upper MFI (+15%)
FL9 – Pacific Blue™ 379,874 446,911 513,948
FL10 – OC515 278,720 327,906 377,092
FL1 – FITC 117,719 138,493 159,267
FL2 – PE 101,813 119,780 137,747
FL4 – PerCPCy™5.5 253,075 297,735 342,395
FL5 – PECy™7 22,648 26,645 30,642
FL6 – APC 637,655 750,182 862,709
AFL8 – APC-C750 162,326 190,972 219,618

Navios™ and Gallios™ are trademarks of Beckman Coulter Inc. Pacific Blue™ is a trademark of Life Technologies. Cy™ is a trademark of GE Healthcare.
EuroFlow™ is a trademark of European Scientific foundation for Laboratory Hemato Oncology (ESLHO).

 

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