ELISAs | Single Analyte Assays
ELISA (Enzyme-linked immunosorbent assay) is a simple, cost-effect technique performed on serum, plasma, cell supernatant, and other biological fluids to determine the presence of an antigen in a sample.
Sandwich ELISAs utilize multiple-well microtiter plates, coated with capture antibodies, to capture soluble proteins. The bound proteins are then detected with a subsequent detection antibody, which is typically labeled with an enzyme, or biotinylated and then followed with streptavidin-enzyme conjugate. A colorimetric substrate is then added, which results in a color change based on the amount of antigen captured. By using a plate reader and plotting resulting values on a standard curve, precise, quantitative values can be obtained.
BioLegend offers a variety of high quality, cost-effective ELISA kits and sets to measure cytokines, chemokines, and soluble biomarkers consistently and reliably. From our easy-to-use, all-inclusive LEGEND MAX™ kits to our ELISA MAX™ Standard sets that provide the core reagents, our selection of ELISA products meets the demand of ELISA beginners to experts alike at a very economical price.
High Accuracy and Sample Linearity of Dilution
The ability to accurately measure different quantities of a standard spiked into sample matrices describes the recovery characteristics of an ELISA. We collect recovery data for all LEGEND MAX™ Kits, typically tested in serum, plasma, and cell culture supernatant.
LEGEND MAX™ Kits also provide linearity results, indicating that at different dilutions of a sample, the results are linear with the expected concentration.
Biological Validation Data Provided
LEGEND MAX™ Kit manuals contain results from testing of in vitro biological samples, using biologically relevant conditions in which the expected cytokine or chemokine is produced. Normal serum/plasma values are also provided.
LEGEND MAX™ Kits and ELISA MAX™ Sets are designed for the quantification of proteins in cell culture supernatant, serum, plasma, and other bodily fluids.
|Assay Buffers||Cat #|
|ELISA Coating Buffer (5X)||421701|
|ELISA Wash Buffer (20X)||421601|
|ELISA Assay Diluent (5X)||421203|
|ELISA Assay Diluent B (5X)||421205|
|TMB High Sensitivity Substrate Solution||421501|
|TMB Substrate Set||421101|
|Stop Solution for TMB Substrate||423001|
|Nunc™ MaxiSorp™ ELISA Plates, Uncoated||423501|