Magnetic Cell Seperation - MojoSort™
MojoSort™ is BioLegend’s magnetic cell separation system for the isolation and purification of cells from heterogeneous populations. MojoSort™ offers outstanding performance at an excellent price.
MojoSort™ Nanobeads are magnetic particles directly conjugated to antibodies (positive selection) or Streptavidin. MojoSort™ Isolation Kits typically contain a biotin-antibody cocktail and Streptavidin Nanobeads, intended to isolate an untouched cell population
Applications
MojoSort™ Isolation Kits Provide Excellent Purity
MojoSort™ Mouse CD4 T Cell Isolation Kit
A single cell suspension from pooled C57BL/6 mouse spleen and lymph nodes was prepared to isolate CD4+ T cells using the MojoSort™ Mouse CD4 T Cell Isolation Kit. Cells were stained with PE anti-mouse CD4 (clone RM4-4), APC anti-mouse CD3ε (145-2C11), and 7-AAD. Dead cells were excluded from the analysis.
MojoSort™ Human CD4 T Cell Isolation Kit
Human PBMC were pooled from three healthy donors to isolate CD4+ T cells using the MojoSort™ Human CD4 T Cell Isolation Kit. Cells were stained with PE anti-CD4 Antibody (clone RPA-T4), APC anti-mouse CD3 (UCHT1), and 7-AAD.
In Vivo Data
CX3CR1+ Cells Migrate Back to the Bone Marrow After Adoptive Transfer
CX3CR1+ cells were isolated from C57BL/6 mice bone marrow using a two step separation protocol. Total bone marrow cells were incubated with directly conjugated Ly-6G Nanobeads to deplete granulocytes. After depletion, the resulting fraction was incubated with biotin anti-mouse CX3CR1 antibody followed by MojoSort™ Streptavidin Nanobeads, resulting in a population containing about 70% of CX3CR1-expressing cells. The cells were then labeled with CFSE and injected back into recipient C57BL/6 mice. Three days later, CFSE+ cells are found in the bone marrow of the recipient mice. Control mouse represents an animal injected with PBS.