Apoptoz Kitleri

Apoptosis Assays

Detection of programmed cell death or apoptosis by Flow Cytometry

  • Highly sensitive assays to detect early to late apoptotic stages;

  • Large number of assays to detect different parameters;

  • Very well validated in Flow Cytometry with many different type of cells;

  • Validated with many different types of drugs;

  • Different fluorescent options for multiple laser excitation sources.

Apoptosis is aregulated process of cell death that occurs during embryonic development as well as maintenance of tissue homeostasis. The appearance of non-regulated apoptosis involves different diseases, such as neurodegenerative diseases and cancer.

The human vascular anticoagulant, Annexin V is a 35-36KDa Ca2+ dependent phospholipids binding protein that has a high affinity for PS. In healthy cells, PS is located on the cytoplasmic surface of the plasma membrane.

However, during early apoptosis, phosphatidylserine (PS), normally located on the cytoplasmic surface of the cell membrane, becomes exposed to the extracellular environment, causing changes in the membrane asymmetry and permeability.

Mitochondrial Assays: detection of early apoptotic cells

The dysfunction of the mitochondria is a special feature of earlier stages of apoptosis. MitoStep apoptosis kits measure changes in the mitochondrial membrane potential with a solution of the cationic cyanine dye DiIC1(5).

In healthy cells, especially in mitochondria with active membrane potentials, the dye accumulates. During apoptosis, the DiIC1(5) fluorescence intensity decreases and thus there is an increase of cells with lower DiIC1(5) fluorescence.

Catalog Number Name Size
ANXVKF-100T FITC Apoptosis Detection Kit 100 test
ANXVKF7-100T FITC Apoptosis Detection Kit 100 test
ANXVKPE-100T PE Apoptosis Detection Kit 100 test
ANXVKDY-100T DY-634 Apoptosis Detection Kit 100 test
ANXVKCFB-100T CF-Blue™ Apoptosis Detection Kit 100 test
ANXVKCFB7-100T CF-Blue™ Apoptosis Detection Kit 100 test
MITO-100T MitoStep 100 test
KMAF-100T FITC MitoStep + Apoptosis Detection Kit 100 test
KMAPE-100T PE MitoStep + Apoptosis Detection Kit 100 test
ANXVF-200T FITC Annexin V 200 test
ANXVPE-200T PE Annexin V 200 test
ANXVDY-200T DY-634 Annexin V 200 test
ANXVCFB-200T CF-Blue™ Annexin V 200 test
ANXVB-200T Biotin Annexin V 200 test
BB10X-50ML Binding Buffer 50 test

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