Exosome Kit ve Ürünleri

Exosomes

Exosomes are small (~40-150 nm) extracellular vesicles (EVs) released from all cell types and found in body fluids and cell culture supernatants. They are generated by fusion of a specialized endosome, the multivesicular body (MVB), with the plasma membrane.

Exosomes have been proposed to provide means for intercellular exchange of macromolecules, allowing the transfer of proteins, lipids, mRNA and miRNA, contributing to intercellular communication in relevant biological processes, including apoptosis, antigen presentation, angiogenesis, inflammation, and coagulation.

ExoStepTM KIT: The most accurate method of exosome detection developed to date.

ExoStepTM kit is the most accurate method of exosome isolation developed to date, a superior alternative for the sensitive detection of exosomes compared with the most commonly used methods. Our kit is easy to implement and analyse for any laboratory that has access to a conventional flow cytometer.

Bead-based assay for Flow Cytometry

ExoStepTM  Kit for the Detection and Characterization of exosomes by Flow Cytometry is composed of an exosome capture reagent consisting of 6 µm diameter magnetic beads coated with a specific antibody as solid suport detectable by the cytometer. Additionally, the capture beads are dyed red and fluorescently labelled which facilitate their detection in the fluorescent detector of a conventional flow cytometer. ExoStepTM contains a detector antibody fluorescent conjugated for exosome detection.

Catalog Number Name Size
ExoS-25-C9 ExoStep  Culture (CD63CB+CD9PE) 25 test
ExoS-25-U9 ExoStep  Urine (CD63CB+CD9PE) 25 test
ExoS-25-P81 ExoStep Plasma (CD9CB+CD81PE) 25 test
ExoS-25-G81 ExoStep  (CD63CB+CD81B+STP-PE) 25 test
MO2ExoS-25-C9 ExoStep Mouse (mCD63CB+mCD9B+STP-PE) 25 test
ExoS-25-G9 ExoStep  (CD63CB+CD9B+STP-PE) 25 test
ExoS-50-CST9 ExoStep  Culture + Standard 50 test
ExoS-50-PST81 ExoStep  Plasma + Standard 50 test

 

Exosome Isolation Columns (SEC)

The most suitable method to isolate exosomes from biological samples

Size Exclusion Chromatography (SEC) has been described as the most efficient method for isolating EVs from complex biological fluids in one single step, with a good recovery and with almost complete removal of contaminants, such as proteins and lipoproteins. At Immunostep, we have developed 70nm and 35nm SEC columns for EVs isolation from fluids such as: plasma, serum, urine and cerebrospinal fluid.

Catalog Number Name Size
SEC7012-4  Evs SEC 70 nm – 4 Pack 4 Column
SEC7012-8  Evs SEC 70 nm – 8 Pack 8 Column
SEC3512-4  Evs SEC 35 nm – 4 Pack 4 Column
SEC3512-8  Evs SEC 35 nm – 8 Pack 8 Column

 

Exosome Capture Beads

Elevate your Exosomes Isolation to the next level with Immunostep’s superparamagnetic Capture Beads:

Specific exosome isolation from different purification tecniques;

Without previous enrichment;

Fully compatible with downstream analysis (WB, mRNA, miRNA, …)

Catalog Number Name Size
IGG2ACB-25 Human IgG2a Capture Beads (Neg. Crtl.) 25 test
IGG1CB-25 Human IgG1 Capture Beads for Flow (Neg. control) 25 test
9CB-25 Human CD9 Capture Beads for Flow Detection 25 test
63CB-25 Human CD63 Capture Beads for Flow Detection 25 test
MO63CB-25 Mouse CD63 Capture Beads for Flow Detection 25 test
81CB-25 Human CD81 Capture Beads for Flow Detection 25 test
274CB-25 Human CD274 Capture Beads for Flow Detection 25 test
326CB-25 Human CD326 (EpCAM) Capture Beads for Flow Cyt. 25 test

 

Exosome Precipitation Solutions

Increase your biomarker sensitivity detection with this simple and fast method:

Ultracentrifugation-free method for a highly-sensitive biomarker detection;

Clean Exosome preparation: reduce carry-over of albumins and immunoglobulins;

Obtain intact exosomes suitable for a wide variety of protein-sensitive applications.

Catalog Number Name Size
EPStep Exosome Precipitation Solution 1 test
EPStep-T Exosome precipitation from Plasma + Thrombin 1 test
EPStep-PS Exosome Precipitation Solution from Plasma – Serum 1 test
EDStep Exosome Detachment Solution 10 ml
EIStep Exosome incubation buffer 15 ml

 

Lyophilized Exosome Standards 

The purest lyophilized standards from human biofluids and different cell culture media

Lyophilization is a useful technique that can be used for long-term stable preservation of exosomes. Besides, lyophilized exosomes can be used as control standards for FACS, WB, ELISA and other applications, as well as quantitative calibration standards for foreign and domestic exosomes in biological samples.

Immunostep Lyophilized Exosome Standards can be used as control for multiple applications, as calibration standards for biomarkers from biological samples and for immunocapture performance evaluation.

Catalog Number Name Size
ExoMSC MSCs-derived exosomes 100 test
ExoSERUM Lyophilized Exosome from Serum 100 test
ExoHT29 Lyophilized Exosome Standards HT29 100 test
ExoMCF7 Lyophilized Exosome Standards MCF7 100 test
ExoA375 Lyophilized Exosome Standards A375 100 test
ExoRPMI Lyophilized Exosome from  RPMICell line Standards 100 test
ExoA549 Lyophilized Exosome from A-549 cell line Standar 100 test
ExoPC3 Lyophilized Exosome Standards PC3 100 test
ExoCaco2 Lyophilized Exosome from Caco2 cell line Standards 100 test
ExoPANC1 Lyophilized Exosome Standards  PANC-1 100 ml
ExoB16F10 Lyophilized Exosome Standards B16F10 100 ml

 

ExoELISA-Step Kit

ExoELISA-Step is an enzyme immunoassay for the detection and quantification of exosomes, based on the use of antigens or antibodies labeled with an enzyme, so that the resulting conjugates have both immunological and enzymatic activity. As one of the components (antigen or antibody) is labeled with an enzyme and insolubilized on a support (immunosorbent), the antigen-antibody reaction will remain immobilized and therefore, is easily revealed by the addition of a specific substrate. When acting on the enzyme a simple or quantifiable view will produce an observable color using a spectrophotometer or a colorimeter.

Catalog Number Name Size
Exo2506 ExoELISA-Step for exosomes from cell culture 96 test
Exo2508 ExoELISA-Step for exosomes from human serum/plasma 96 test

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