Analysis of white blood cell infiltration of Cerebrospinal Fluid (CSF) is important in the diagnosis of diseases including inflammatory neurologic diseases, acute and chronic leukaemia, myelodysplastic syndromes, lymphomas, myeloma, autoimmune diseases, immunodeficiencies and solid tumours. Flow cytometry is an important method for diagnosis, classification and monitoring of these diseases. However, cells within untreated CSF samples are typically low in number and degrade rapidly ex vivo, resulting in low sensitivity of disease detection.

Stabilisation of CSF with TransFix CSF Sample Storage Tubes provides increased recovery of myeloid and lymphoid cells for up to 72 hours for analysis via flow cytometry, compared to untreated or cell culture medium treated CSF^(1,2).

Benefits of TransFix CSF Sample Tubes

• Increased disease detection – early diagnosis and treatment for better patient outcome
• Reduction in the need for repeat lumbar puncture – reduced costs and trauma for patients
• Provides the opportunity to transport the samples for centralised diagnosis and multicentre clinical studies
• Easy to use – just transfer 1-4 mL of CSF sample into the tube within 1 hour of lumbar puncture and mix by inversion.
• Provides greater flexibility for the laboratory – eliminates the need for weekend and evening work and reduces the impact of unexpected machine breakdown or staff shortages.
• Recommended in the guidelines published in the British Journal of Haematology ^(3,4) and endorsed by European Society for Clinical Cell Analysis and the Italian Society for Clinical Cell Analysis ⁽⁵⁾, the Spanish Lymphoma Group ⁽⁶⁾, Spanish Group for the Study of CNS Disease in Non-Hodgkins Lymphoma ⁽⁷⁾, Brazilian Group of Flow Cytometry ⁽⁸⁾, International Working Group on Flow Cytometric Immunophenotyping of Cerebrospinal fluid ⁽⁹⁾, Colombian Consensus of FCM ⁽¹⁰⁾.

References

1. de Jongste AH, Kraan J, van den Broek PD, Brooimans RA, Bromberg JE, van Montfort KA, Smitt PAS, and Gratama JW. Use of TransFix™ Cerebrospinal Fluid Storage Tubes Prevents Cellular Loss and Enhances Flow Cytometric Detection of Malignant Hematological Cells After 18 Hours of Storage. Cytometry Part B 2014; 86B: 272– 279. https://doi.org/10.1002/cyto.b.21097
2. Kaenzig et al. Evaluation of TransFix CSF Sample Storage Tubes compared to alternative preservation methods (Caltag Medsystems Ltd 2022- unpublished)
3. Johansson U, Bloxham D, Couzens S, Jesson J, Morilla R, Erber W, and Macey M. (2014). Guidelines on the use of multicolour flow cytometry in the diagnosis of haematological neoplasms. British Journal of Haematology, 165, 455–488.
4. Baraniskin A, Deckert M, Schulte-Altedorneburg G, Schlegel U, Schroers R. Current strategies in the diagnosis of diffuse large B-cell lymphoma of the central nervous system. Br J Haematol. 2012 Feb;156(4):421-32. doi: 10.1111/j.1365-2141.2011.08928
5. Del Principe MI, Gatti A, Johansson U, Buccisano F, Brando B. ESCCA/ISCCA protocol for the analysis of cerebrospinal fluid by multiparametric flow‐cytometry in hematological malignancies. Cytometry. 2020; 1– 13. https://doi.org/10.1002/cyto.b.21981
6. Peñalver FJ, Sancho JM, de la Fuente A, Olave MT, Martín A, Panizo C, Pérez E, Salar A, Orfao A. Guidelines for diagnosis, prevention and management of central nervous system involvement in diffuse large B-cell lymphoma patients by the Spanish Lymphoma Group (GELTAMO). Haematologica 2017;102(2):235-245; https://doi.org/10.3324/haematol.2016.149120
7. Quijano S, López A, Manuel Sancho J, Panizo C, Debén G, Castilla C, Antonio García-Vela J, Salar A, Alonso-Vence N, González-Barca E, Peñalver FJ, Plaza-Villa J, Morado M, García-Marco J, Arias J, Briones J, Ferrer S, Capote J, Nicolás C, Orfao A; Spanish Group for the Study of CNS Disease in NHL. Identification of leptomeningeal disease in aggressive B-cell non-Hodgkin’s lymphoma: improved sensitivity of flow cytometry. J Clin Oncol. 2009 Mar 20;27(9):1462-9. doi: 10.1200/JCO.2008.17.7089.
8. Correia RP, Bortolucci ACA, Lopes ACW, Sandes AF, Azambuja AP, Viana MA, et al. Recommendations for quality assurance in multiparametric flow cytometry: first concensus of the Brazilian Group of Flow Cytometry (GBCFLUX). J. Bras. Patol. Med. Lab.2015;51(6):389-396 https://doi.org/10.5935/1676-2444.20150061
9. Kraan J, Gratama JW, Haioun C, Orfao A, Plonquet A, Porwit A, Quijano S, Stetler-Stevenson M, Subira D and Wilson W. (2008), Flow Cytometric Immunophenotyping of Cerebrospinal Fluid. Current Protocols in Cytometry, 45: 6.25.1-6.25.16. https://doi.org/10.1002/0471142956.cy0625s45
10. Campos A, Trujillo L, López D, Beltrán L, Arias E, Vélez G, Infante A, Reyes I, Vizcaíno M, Guzmán P C, Herrera MV, Solano J, Londono D, Cañas A, Pretelt F, Pérez JC, Cardozo C, Fiorentino S, and Quijano, S. (2017). Study of body fluid samples using flow cytometry: Six years of experience at the Hospital Universitario San Ignacio – Pontificia Universidad Javeriana, Bogota – Colombia. Universitas Scientiarum (2017). 22. 123-143. 10.11144/Javeriana.SC22-2.sobf.

 

Product Information

Product Code	Description	Quantity
TF-CSF-L-2	TransFix CSF 1-4 mL Sample Storage Tube (2 tubes)	2 Tubes
TF-CSF-L-10	TransFix CSF 1-4 mL Sample Storage Tube (10 tubes)	10 Tubes
TF-CSF-L-50	TransFix CSF 1-4 mL Sample Storage Tube (50 tubes)	50 Tubes
TF-CSF-S-2	TransFix CSF 0.25-1 mL Sample Storage Tube (2 tubes)	2 Tubes
TF-CSF-S-10	TransFix CSF 0.25-1 mL Sample Storage Tube (10 tubes)	10 Tubes
TF-CSF-S-50	TransFix CSF 0.25-1 mL Sample Storage Tube (50 tubes)	50 Tubes