The Vena8 Endothelial+™ biochip is ideal for cell-cell rolling and adhesion assays on cell monolayers and cell culture under shear flow.  Ideal for use with PCR.  Compatible with brightfield, phase contrast imaging and fluorescence microscopy.

Features:

—Cell-cell rolling and adhesion assays on cell monolayers and cell culture under shear flow.

—Cells cultured within the biochip channels may be retrieved post-assay for subsequent studies (up to 320,000 cells); e.g. PCR.  Substrate is removed from biochip and cells are scraped off.

—Suitable for whole blood, primary cells, rare cells e.g. where samples are more difficult to retrieve or culture or only small sample volumes available.

—No assembly of the biochip is required unlike many standard perfusion chambers / flow chambers.

—No luer lock connections which increase dead volume.  Cellix’s biochips have a unique plug & play connection with tubing connections which are autoclaveable and reuseable.  Dead volume is at input port ~ 0.1μL.

Applications:  Flow assays using minimal sample volumes (whole blood, cell suspensions, proteins etc.); brightfield, phase contrast and fluorescence microscopy.

Specifications:

Substrate thickness	0.5mm = 500μm
Substrate material	High quality plastic, compatible with brightfield, phase contrast imaging and fluorescence microscopy
Number of channels per biochip	8
Height of channels	0.12mm = 120μm
Width of channels	0.8mm = 800μm
Length of channels	28mm = 2.8cm
Volume of each channel	2.69μL
Volume of protein required for coating	12μL (e.g. fibronectin to promote adhesion of cells for culture)
Volume of cell sample required for seeding	5μL; cell concentration = 15 x 106 cells/mL
Growth area per channel	0.096 mm2
Typical number of HUVECs cultured per channel	~ 40,000 cells per channel
Typical number of cells retrieved from one chip	~ 320,000 cells per chip
Volume of reservoir to hold sample	0.5mL; also possible to connect via tubing to eppendorf if greater sample volume or high shear stress / flow rate assay is being performed