The Vena8™ biochip with/without glass coverslip adhered; with/without treatment for cell rolling and adhesion on proteins; seeding of cells or microbes and subsequent study of drug interaction, imaging or molecular biology studies under shear flow. Compatible with brightfield, phase contrast imaging, immunofluorescence and confocal microscopy.
• Cell rolling and adhesion on proteins; seeding of cells or microbes and subsequent study of drug interaction, imaging or molecular biology studies under shear flow.
• Biochips are supplied with or without glass coverslip attached.
• Glass coverslip may be treated or non-treated; e.g. glass may be treated with chelated Cu2+ ion tethered to the high-density PEG coating – surface of the microchannels may then be easily coated via pipetting and the protein binds via the poly-histidine tag.
• Biochips available for low or high flow rates / shear stresses.
• Ideal for whole blood, primary cells, rare cells e.g. where samples are more difficult to retrieve or culture or only small sample volumes available.
• Perfect for high flow rates / high shear stresses e.g. platelet adhesion, aggregation and thrombi formation studies for thrombosis using whole blood. In standard flow chambers / perfusion chambers; significant volumes of blood would be required making the assay impossible.
• Suitable for biofilm applications; particularly Vena8 biochips supplied without glass coverslip: user may attach any type of coupon to bottom of the biochip.
• No assembly of the biochip is required unlike many standard perfusion chambers / flow chambers.
• No luer lock connections which increase dead volume. Cellix’s biochips have a unique plug & play connection with tubing connections which are autoclaveable and reuseable.
Applications: Flow assays using minimal sample volumes (whole blood, cell suspensions, proteins etc.); immunofluorescence and confocal microscopy.
|Substrate thickness||0.17mm = 170μm|
|Substrate material||Glass, compatible with immunofluorescence and confocal microscopy|
|Number of channels per biochip||8|
|LOW FLOW RATE BIOCHIPS:|
|Channel dimensions||160μm (H) x 1600μm (W) x 28mm ≡ 0.16mm (H) x 1.6mm (W) x 28mm|
|Volume of each channel||7.168μL|
|Volume of protein required for coating||~20μL|
|HIGH FLOW RATE BIOCHIPS:|
|Channel dimensions||80μm (H) x 800μm (W) x 28mm ≡ 0.08mm (H) x 0.8mm (W) x 28mm|
|Volume of each channel||1.79μL|
|Volume of protein required for coating||10μL|
|Volume of reservoir to hold sample||0.5mL; also possible to connect via tubing to eppendorf if greater sample volume or high shear stress / flow rate assay is being performed|
|Thrombosis assays – recommendations for whole blood:||• Biochips: Vena8 with Glass Coverslips Adhered, Treated with Cu2+ histidine tag; Low Flow Rates; Pack 10 or Vena8 with Glass Coverslips Adhered, Treated with Cu2+ histidine tag; High Flow Rates; Pack 10
• Pumps: ExiGo or Mirus Evo Nanopump
• Low flow rates / shear stresses: 0.45 – 9 dyne/cm2
• High flow rates / shear stresses: 90 – 180 dyne/cm2
|Stem cell assays – recommendations for stem cell culture:||• Biochips: Vena8 with Glass Coverslips Adhered, Non-treated; Low Flow Rates; Pack 10
• Pumps: Kima pump
• Low flow rates / shear stresses: 1 dyne/cm2
|Biofilm assays – recommendations for biofilm culture:||• Biochips: Vena8 with adhesive bottom layer; Low Flow Rates; Pack 10; biochips have a protective cover which can be peeled away to reveal adhesive bottom layer to which any material may be attached by the user.
• Pumps: Kima pump
• Low flow rates / shear stresses: 1 dyne/cm2; particularly suited for biofilm applications with Kima pump achieving shear stresses of 1dyne/cm2where different substrate materials may be easily tested.
iPod Touch Kima Pump for Biofilm Culture studies
**NO AUDIO WITH THIS VIDEO** The Kima Pump is a simple to use, iPod Touch controlled microfluidic pump for life science applications including: aiding cell culture (e.g. endothelial cells) under physiological conditions (shear flow) in biochips and flow/perfusion chambers; electrophysiology experiments; biofilm culture; investigation of nanoparticle uptake in cells and long-term microfluidic toxicology studies. This movie shows the application of iPod Touch controlled Kima pump for biofilm culture studies. The Kima pump connects easily to Cellix\’s biochips and other manufacturer\’s flow chambers via fully autoclaveable tubing. The iKima App will shortly be available for download from Apple\’s iTunes Store.