Transfer sponge pads for more reproducible Western blot transfers
• Resist compression – maintain proper pressure in transfer sandwiches without adding extra blotting papers
• Retain thickness – can be reused over multiple experiments
• Convenient – two sizes (8 x 11 cm and 9 x 15 cm) compatible with a variety of transfer apparatuses
• Standardized – uniform flat construction ensures reproducible and consistent transfers
Advansta’s Blotting sponge pads maintain secure contact between gel and membrane in transfer sandwiches and cassettes for consistent transfers. The blotting sponge pads resist compression and are available in two sizes (8 x 11 cm and 9 x 15 cm) to work with multiple transfer setups and membrane sizes.
Figure 1: The below diagram demonstrates the role of sponge pads in the transfer sandwich set-up, outside the blotting papers and just inside the transfer cassette. Sponge pads are important for making sure equal pressure is applied for even transfer from gel to membrane.