A chemiluminescent marker for writing on membranes
• Improve accuracy – transform your visible protein ladder standard into a chemiluminescent standard on your Western blot
• Annotate – permanently mark your blots with the date or blot ID
• Compatible – with both X-ray film and CCD imagers
• Confirm HRP substrate stability – the ChemiPen reagent glows when incubated with an HRP substrate, so it can be used to test substrate stability
Write or draw on your transfer membranes with the WesternBright ChemiPen. The reagent in the ChemiPen adsorbs to nitrocellulose and PVDF membranes, and reacts with HRP substrates to produce chemiluminescence that can be detected with X-ray film or CCD imaging.
With the proprietary “ink” you can transform your visible protein markers into chemiluminescent markers, to annotate your blot with a date or blot ID, or to check the stability of your HRP substrate (Figure 1).
Figure 1. The WesternBright ChemiPen can be used to mark the location of protein standards and to annotate the blot with a date, as in the right panel.
The WesternBright ChemiPen has two tips, a fine tip at one end that is perfect for writing on the blot or tracing the bands of a visible protein standard, and a thicker wedge tip that can be used to deposit greater amounts of reagent on the blot. Marking the location of visible protein standards on a blot is easy. Simply trace the visible markers on the membrane after transfer and before blocking (Figure 2).
Figure 2. Use the WesternBright ChemiPen to visualize visible protein standards on chemiluminescent Western blots