Convenient nitrocellulose and PVDF membranes for Western blotting
• Performance – quality controlled for background-free Western blots
• Convenience – membranes available pre-cut and as rolls
• High signal-to-noise ratio – low background signal for clearer results
• Flexibility – choose the right membrane for your Western blotting application
WesternBright PVDF-FL: fluorescent detection
WesternBright PVDF-CL: chemiluminescent detection; best stability for stripping and re-probing chemiluminescent blots
PVDF membranes available as rolls or pre-cut (7×9 cm, 10×15 cm, or 13×18 cm)
WesternBright NC: all-purpose nitrocellulose membrane
Nitrocellulose membranes available as rolls or pre-cut (8×10 cm)
Advansta offers a large selection of nitrocellulose and polyvinylidene difluoride (PVDF) transfer membranes for immunoblotting applications. Choose from convenient pre-cut membranes sized for protein minigels and economical membrane rolls. All membranes are optimized for compatibility with a wide range of protein stains and enzyme-based detection systems, specifically with chemiluminescent substrates.
The WesternBright PVDF membranes provide durability and high tensile strength for ease of handling. Advansta offers PVDF membranes appropriate for chemiluminescent detection (PVDF-CL) and low-autofluorescence PVDF membranes (PVDF-FL) perfect for fluorescent detection.
The WesternBright nitrocellulose membranes are pure nitrocellulose media that has high binding capacity for proteins. These nitrocellulose membranes are stronger and more durable than other nitrocellulose membranes, and will not rip, tear, or crack during transfers. The nature of this material yields sharper images with little or no distortion.
Pre-cut membranes are compatible with gels 10 x 10 cm and smaller, including:
|Bio-Rad||Mini-PROTEAN® Tetra system, Mini-PROTEAN and Ready Gel® precast gels|
|GE Healthcare||miniVE system, Hoefer™ SE 250 and SE 260 Mighty Small II systems|
|Invitrogen||NuPAGE® Novex® and Novex ZOOM® precast gels, XCell6™ MultiGel system, XCell Surelock™ system|
|Pierce||Precise™ Protein Gels|
|Thermo Electron||EC120 Mini Vertical Gel System|